Two-Hybrid System Techniques
                             
                            
                            
                                
                            
                            
                                
                            
                            
                            
                                
                                    
                                            
	"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, 
	MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, 
	which enables searching at various levels of specificity.
	
	
		
			
			
				Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
    
			
			
				
				
					
						| Descriptor ID | D020798 | 
					
						| MeSH Number(s) | E05.393.220.870 E05.601.690.650 E05.601.870 | 
					
						| Concept/Terms | Two-Hybrid System TechniquesTwo-Hybrid System TechniquesTechnique, Two-Hybrid SystemTechniques, Two-Hybrid SystemTwo Hybrid System TechniquesTwo-Hybrid System TechniqueTwo-Hybrid MethodMethod, Two-HybridMethods, Two-HybridTwo Hybrid MethodTwo-Hybrid MethodsTwo-Hybrid System TechnicsTechnic, Two-Hybrid SystemTechnics, Two-Hybrid SystemTwo Hybrid System TechnicsTwo-Hybrid System TechnicTwo-Hybrid AssayAssay, Two-HybridAssays, Two-HybridTwo Hybrid AssayTwo-Hybrid Assays
 One-Hybrid System TechniquesOne-Hybrid System TechniquesOne Hybrid System TechniquesOne-Hybrid System TechniqueTechnique, One-Hybrid SystemTechniques, One-Hybrid SystemOne-Hybrid System TechnicsOne Hybrid System TechnicsOne-Hybrid System TechnicTechnic, One-Hybrid SystemTechnics, One-Hybrid System
 Yeast Two-Hybrid System TechniquesYeast Two-Hybrid System TechniquesYeast Two Hybrid System TechniquesYeast Two-Hybrid SystemSystem, Yeast Two-HybridSystems, Yeast Two-HybridTwo-Hybrid System, YeastTwo-Hybrid Systems, YeastYeast Two Hybrid SystemYeast Two-Hybrid SystemsYeast Two-Hybrid AssayAssay, Yeast Two-HybridAssays, Yeast Two-HybridTwo-Hybrid Assay, YeastTwo-Hybrid Assays, YeastYeast Two Hybrid AssayYeast Two-Hybrid Assays
 Three-Hybrid System TechniquesThree-Hybrid System TechniquesTechnique, Three-Hybrid SystemTechniques, Three-Hybrid SystemThree Hybrid System TechniquesThree-Hybrid System TechniqueTri-Hybrid System TechniquesTechnique, Tri-Hybrid SystemTechniques, Tri-Hybrid SystemTri Hybrid System TechniquesTri-Hybrid System TechniqueThree-Hybrid System TechnicsTechnic, Three-Hybrid SystemTechnics, Three-Hybrid SystemThree Hybrid System TechnicsThree-Hybrid System Technic
 Yeast Three-Hybrid System TechniquesYeast Three-Hybrid System TechniquesYeast Three Hybrid System TechniquesYeast Three-Hybrid SystemSystem, Yeast Three-HybridSystems, Yeast Three-HybridThree-Hybrid System, YeastThree-Hybrid Systems, YeastYeast Three Hybrid SystemYeast Three-Hybrid SystemsYeast Three-Hybrid AssayAssay, Yeast Three-HybridAssays, Yeast Three-HybridThree-Hybrid Assay, YeastThree-Hybrid Assays, YeastYeast Three Hybrid AssayYeast Three-Hybrid Assays
 n-Hybrid System Techniquesn-Hybrid System TechniquesSystem Technique, n-HybridSystem Techniques, n-HybridTechnique, n-Hybrid SystemTechniques, n-Hybrid Systemn Hybrid System Techniquesn-Hybrid System Technique
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				Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".
				
			 
			
			
				Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".
				
			 
		 
	 
 
                                        
                                            
	
	
		
			
			
					
				This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications. 
				
					 
                    To see the data from this visualization as text, 
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		            | Year | Major Topic | Minor Topic | Total | 
|---|
| 2000 | 0 | 4 | 4 | 
| 2001 | 1 | 4 | 5 | 
| 2002 | 0 | 2 | 2 | 
| 2003 | 0 | 6 | 6 | 
| 2004 | 0 | 5 | 5 | 
| 2005 | 0 | 6 | 6 | 
| 2006 | 0 | 3 | 3 | 
| 2007 | 0 | 2 | 2 | 
| 2008 | 0 | 5 | 5 | 
| 2009 | 0 | 1 | 1 | 
| 2010 | 0 | 3 | 3 | 
| 2011 | 0 | 4 | 4 | 
| 2012 | 0 | 2 | 2 | 
| 2013 | 0 | 2 | 2 | 
| 2014 | 0 | 1 | 1 | 
| 2015 | 0 | 1 | 1 | 
| 2017 | 0 | 3 | 3 | 
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				Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.
						
					
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								Base-resolution stratification of cancer mutations using functional variomics. Nat Protoc. 2017 Nov; 12(11):2323-2341. 
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								Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. Proc Natl Acad Sci U S A. 2017 07 11; 114(28):E5530-E5538. 
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								The heterotrimeric G protein G?1 interacts with the catalytic subunit of protein phosphatase 1 and modulates G protein-coupled receptor signaling in platelets. J Biol Chem. 2017 08 11; 292(32):13133-13142. 
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								Pooled-matrix protein interaction screens using Barcode Fusion Genetics. Mol Syst Biol. 2016 Apr 22; 12(4):863. 
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								Grancalcin (GCA) modulates Toll-like receptor 9 (TLR9) mediated signaling through its direct interaction with TLR9. Eur J Immunol. 2016 Mar; 46(3):712-24. 
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								Human gene-centered transcription factor networks for enhancers and disease variants. Cell. 2015 Apr 23; 161(3):661-673. 
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								A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor. Curr Biol. 2015 Mar 02; 25(5):613-20. 
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								Absent, small or homeotic 2-like protein (ASH2L) enhances the transcription of the estrogen receptor a gene through GATA-binding protein 3 (GATA3). J Biol Chem. 2014 Nov 07; 289(45):31373-81. 
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								Systematic identification of pathological lamin A interactors. Mol Biol Cell. 2014 May; 25(9):1493-510. 
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								Plakophilin-3 catenin associates with the ETV1/ER81 transcription factor to positively modulate gene activity. PLoS One. 2014; 9(1):e86784.