TIMOTHY PALZKILL to beta-Lactamases
This is a "connection" page, showing publications TIMOTHY PALZKILL has written about beta-Lactamases.
Connection Strength
22.550
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Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning. Proc Natl Acad Sci U S A. 2024 Mar 19; 121(12):e2313513121.
Score: 0.745
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Mutagenesis and structural analysis reveal the CTX-M ?-lactamase active site is optimized for cephalosporin catalysis and drug resistance. J Biol Chem. 2023 05; 299(5):104630.
Score: 0.696
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Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M ?-lactamase. Commun Biol. 2023 01 12; 6(1):35.
Score: 0.687
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Evaluation of Tebipenem Hydrolysis by ?-Lactamases Prevalent in Complicated Urinary Tract Infections. Antimicrob Agents Chemother. 2022 05 17; 66(5):e0239621.
Score: 0.655
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Deep Sequencing of a Systematic Peptide Library Reveals Conformationally-Constrained Protein Interface Peptides that Disrupt a Protein-Protein Interaction. Chembiochem. 2022 02 04; 23(3):e202100504.
Score: 0.637
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Local interactions with the Glu166 base and the conformation of an active site loop play key roles in carbapenem hydrolysis by the KPC-2 ?-lactamase. J Biol Chem. 2021 Jan-Jun; 296:100799.
Score: 0.613
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Mechanistic Basis of OXA-48-like ?-Lactamases' Hydrolysis of Carbapenems. ACS Infect Dis. 2021 02 12; 7(2):445-460.
Score: 0.600
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KPC-2 ?-lactamase enables carbapenem antibiotic resistance through fast deacylation of the covalent intermediate. J Biol Chem. 2021 Jan-Jun; 296:100155.
Score: 0.595
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A drug-resistant ?-lactamase variant changes the conformation of its active-site proton shuttle to alter substrate specificity and inhibitor potency. J Biol Chem. 2020 12 25; 295(52):18239-18255.
Score: 0.590
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Antagonism between substitutions in ?-lactamase explains a path not taken in the evolution of bacterial drug resistance. J Biol Chem. 2020 05 22; 295(21):7376-7390.
Score: 0.568
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Development and Evaluation of a Novel Protein-Based Assay for Specific Detection of KPC ?-Lactamases from Klebsiella pneumoniae Clinical Isolates. mSphere. 2020 01 08; 5(1).
Score: 0.558
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Differential active site requirements for NDM-1 ?-lactamase hydrolysis of carbapenem versus penicillin and cephalosporin antibiotics. Nat Commun. 2018 10 30; 9(1):4524.
Score: 0.514
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Synergistic effects of functionally distinct substitutions in ?-lactamase variants shed light on the evolution of bacterial drug resistance. J Biol Chem. 2018 11 16; 293(46):17971-17984.
Score: 0.511
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The Drug-Resistant Variant P167S Expands the Substrate Profile of CTX-M ?-Lactamases for Oxyimino-Cephalosporin Antibiotics by Enlarging the Active Site upon Acylation. Biochemistry. 2017 07 11; 56(27):3443-3453.
Score: 0.468
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Systematic substitutions at BLIP position 50 result in changes in binding specificity for class A ?-lactamases. BMC Biochem. 2017 03 06; 18(1):2.
Score: 0.458
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BLIP-II Employs Differential Hotspot Residues To Bind Structurally Similar Staphylococcus aureus PBP2a and Class A ?-Lactamases. Biochemistry. 2017 02 28; 56(8):1075-1084.
Score: 0.457
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Engineering Specificity from Broad to Narrow: Design of a ?-Lactamase Inhibitory Protein (BLIP) Variant That Exclusively Binds and Detects KPC ?-Lactamase. ACS Infect Dis. 2016 12 09; 2(12):969-979.
Score: 0.447
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Deep Sequencing of Random Mutant Libraries Reveals the Active Site of the Narrow Specificity CphA Metallo-?-Lactamase is Fragile to Mutations. Sci Rep. 2016 09 12; 6:33195.
Score: 0.443
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Removal of the Side Chain at the Active-Site Serine by a Glycine Substitution Increases the Stability of a Wide Range of Serine ?-Lactamases by Relieving Steric Strain. Biochemistry. 2016 05 03; 55(17):2479-90.
Score: 0.431
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Characterization of the global stabilizing substitution A77V and its role in the evolution of CTX-M ?-lactamases. Antimicrob Agents Chemother. 2015 Nov; 59(11):6741-8.
Score: 0.411
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Natural Variants of the KPC-2 Carbapenemase have Evolved Increased Catalytic Efficiency for Ceftazidime Hydrolysis at the Cost of Enzyme Stability. PLoS Pathog. 2015 Jun; 11(6):e1004949.
Score: 0.405
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How structural and physicochemical determinants shape sequence constraints in a functional enzyme. PLoS One. 2015; 10(2):e0118684.
Score: 0.398
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A triple mutant in the O-loop of TEM-1 ?-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis. J Biol Chem. 2015 Apr 17; 290(16):10382-94.
Score: 0.398
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Role of ?-lactamase residues in a common interface for binding the structurally unrelated inhibitory proteins BLIP and BLIP-II. Protein Sci. 2014 Sep; 23(9):1235-46.
Score: 0.380
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Metallo-?-lactamase structure and function. Ann N Y Acad Sci. 2013 Jan; 1277:91-104.
Score: 0.340
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Deep sequencing of systematic combinatorial libraries reveals ?-lactamase sequence constraints at high resolution. J Mol Biol. 2012 Dec 07; 424(3-4):150-67.
Score: 0.337
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Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-?-lactamase active site. Antimicrob Agents Chemother. 2012 Nov; 56(11):5667-77.
Score: 0.334
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Analysis of the functional contributions of Asn233 in metallo-?-lactamase IMP-1. Antimicrob Agents Chemother. 2011 Dec; 55(12):5696-702.
Score: 0.313
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Use of periplasmic target protein capture for phage display engineering of tight-binding protein-protein interactions. Protein Eng Des Sel. 2011 Nov; 24(11):819-28.
Score: 0.313
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Analysis of the binding forces driving the tight interactions between beta-lactamase inhibitory protein-II (BLIP-II) and class A beta-lactamases. J Biol Chem. 2011 Sep 16; 286(37):32723-35.
Score: 0.310
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Multiple global suppressors of protein stability defects facilitate the evolution of extended-spectrum TEM ?-lactamases. J Mol Biol. 2010 Dec 17; 404(5):832-46.
Score: 0.294
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Structural and biochemical evidence that a TEM-1 beta-lactamase N170G active site mutant acts via substrate-assisted catalysis. J Biol Chem. 2009 Nov 27; 284(48):33703-12.
Score: 0.274
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Analysis of the plasticity of location of the Arg244 positive charge within the active site of the TEM-1 beta-lactamase. Protein Sci. 2009 Oct; 18(10):2080-9.
Score: 0.274
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Genetic and structural characterization of an L201P global suppressor substitution in TEM-1 beta-lactamase. J Mol Biol. 2008 Dec 05; 384(1):151-64.
Score: 0.255
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A fitness cost associated with the antibiotic resistance enzyme SME-1 beta-lactamase. Genetics. 2007 Aug; 176(4):2381-92.
Score: 0.233
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Amino acid residues that contribute to substrate specificity of class A beta-lactamase SME-1. Antimicrob Agents Chemother. 2005 Aug; 49(8):3421-7.
Score: 0.205
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Analysis of the context dependent sequence requirements of active site residues in the metallo-beta-lactamase IMP-1. J Mol Biol. 2004 Nov 26; 344(3):653-63.
Score: 0.196
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Dissecting the protein-protein interface between beta-lactamase inhibitory protein and class A beta-lactamases. J Biol Chem. 2004 Oct 08; 279(41):42860-6.
Score: 0.191
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The mechanism of ceftazidime and cefiderocol hydrolysis by D179Y variants of KPC carbapenemases is similar and involves the formation of a long-lived covalent intermediate. Antimicrob Agents Chemother. 2024 03 06; 68(3):e0110823.
Score: 0.185
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Evaluation of penicillin-based inhibitors of the class A and B beta-lactamases from Bacillus anthracis. Biochem Biophys Res Commun. 2004 Jan 16; 313(3):541-5.
Score: 0.184
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Exploiting the Carboxylate-Binding Pocket of ?-Lactamase Enzymes Using a Focused DNA-Encoded Chemical Library. J Med Chem. 2024 01 11; 67(1):620-642.
Score: 0.183
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Klebsiella pneumoniae carbapenemase variant 44 acquires ceftazidime-avibactam resistance by altering the conformation of active-site loops. J Biol Chem. 2024 01; 300(1):105493.
Score: 0.182
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Determinants of binding affinity and specificity for the interaction of TEM-1 and SME-1 beta-lactamase with beta-lactamase inhibitory protein. J Biol Chem. 2003 Nov 14; 278(46):45706-12.
Score: 0.179
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Biochemical characterization of beta-lactamases Bla1 and Bla2 from Bacillus anthracis. Antimicrob Agents Chemother. 2003 Jun; 47(6):2040-2.
Score: 0.176
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Amino acid sequence requirements at residues 69 and 238 for the SME-1 beta-lactamase to confer resistance to beta-lactam antibiotics. Antimicrob Agents Chemother. 2003 Mar; 47(3):1062-7.
Score: 0.173
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An active site loop toggles between conformations to control antibiotic hydrolysis and inhibition potency for CTX-M ?-lactamase drug-resistance enzymes. Nat Commun. 2022 11 07; 13(1):6726.
Score: 0.170
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Molecular analysis of beta-lactamase structure and function. Int J Med Microbiol. 2002 Jul; 292(2):127-37.
Score: 0.166
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Consensus on ?-Lactamase Nomenclature. Antimicrob Agents Chemother. 2022 04 19; 66(4):e0033322.
Score: 0.163
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Identification of residues critical for metallo-beta-lactamase function by codon randomization and selection. Protein Sci. 2001 Dec; 10(12):2556-65.
Score: 0.159
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Unique Diacidic Fragments Inhibit the OXA-48 Carbapenemase and Enhance the Killing of Escherichia coli Producing OXA-48. ACS Infect Dis. 2021 12 10; 7(12):3345-3354.
Score: 0.159
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QM/MM modeling of class A ?-lactamases reveals distinct acylation pathways for ampicillin and cefalexin. Org Biomol Chem. 2021 Nov 03; 19(42):9182-9189.
Score: 0.158
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Amino acid sequence determinants of extended spectrum cephalosporin hydrolysis by the class C P99 beta-lactamase. J Biol Chem. 2001 Dec 07; 276(49):46568-74.
Score: 0.157
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Mapping Protein-Protein Interaction Interface Peptides with Jun-Fos Assisted Phage Display and Deep Sequencing. ACS Synth Biol. 2020 07 17; 9(7):1882-1896.
Score: 0.144
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Identifying Oxacillinase-48 Carbapenemase Inhibitors Using DNA-Encoded Chemical Libraries. ACS Infect Dis. 2020 05 08; 6(5):1214-1227.
Score: 0.142
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A Standard Numbering Scheme for Class C ?-Lactamases. Antimicrob Agents Chemother. 2020 02 21; 64(3).
Score: 0.141
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Susceptibility of beta-lactamase to core amino acid substitutions. Protein Eng. 1999 Sep; 12(9):761-9.
Score: 0.136
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Identification of residues in beta-lactamase critical for binding beta-lactamase inhibitory protein. J Biol Chem. 1999 Mar 12; 274(11):6963-71.
Score: 0.132
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The role of residue 238 of TEM-1 beta-lactamase in the hydrolysis of extended-spectrum antibiotics. J Biol Chem. 1998 Oct 09; 273(41):26603-9.
Score: 0.128
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beta-Lactamases: protein evolution in real time. Trends Microbiol. 1998 Aug; 6(8):323-7.
Score: 0.126
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Cephalosporin substrate specificity determinants of TEM-1 beta-lactamase. J Biol Chem. 1997 Nov 14; 272(46):29144-50.
Score: 0.120
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A natural polymorphism in beta-lactamase is a global suppressor. Proc Natl Acad Sci U S A. 1997 Aug 05; 94(16):8801-6.
Score: 0.118
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Selection and characterization of amino acid substitutions at residues 237-240 of TEM-1 beta-lactamase with altered substrate specificity for aztreonam and ceftazidime. J Biol Chem. 1996 Sep 13; 271(37):22538-45.
Score: 0.111
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Amino acid sequence determinants of beta-lactamase structure and activity. J Mol Biol. 1996 May 17; 258(4):688-703.
Score: 0.108
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Systematic mutagenesis of the active site omega loop of TEM-1 beta-lactamase. J Bacteriol. 1996 Apr; 178(7):1821-8.
Score: 0.107
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Structural Basis for Different Substrate Profiles of Two Closely Related Class D ?-Lactamases and Their Inhibition by Halogens. Biochemistry. 2015 Jun 02; 54(21):3370-80.
Score: 0.101
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New variant of TEM-10 beta-lactamase gene produced by a clinical isolate of proteus mirabilis. Antimicrob Agents Chemother. 1995 May; 39(5):1199-200.
Score: 0.101
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Molecular basis for the catalytic specificity of the CTX-M extended-spectrum ?-lactamases. Biochemistry. 2015 Jan 20; 54(2):447-57.
Score: 0.098
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Effect of threonine-to-methionine substitution at position 265 on structure and function of TEM-1 beta-lactamase. Antimicrob Agents Chemother. 1994 Oct; 38(10):2266-9.
Score: 0.097
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Characterization of TEM-1 beta-lactamase mutants from positions 238 to 241 with increased catalytic efficiency for ceftazidime. J Biol Chem. 1994 Sep 23; 269(38):23444-50.
Score: 0.097
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Evolution of antibiotic resistance: several different amino acid substitutions in an active site loop alter the substrate profile of beta-lactamase. Mol Microbiol. 1994 Apr; 12(2):217-29.
Score: 0.093
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Selection of functional signal peptide cleavage sites from a library of random sequences. J Bacteriol. 1994 Feb; 176(3):563-8.
Score: 0.092
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Identification of the ?-lactamase inhibitor protein-II (BLIP-II) interface residues essential for binding affinity and specificity for class A ?-lactamases. J Biol Chem. 2013 Jun 14; 288(24):17156-66.
Score: 0.088
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BLIP-II is a highly potent inhibitor of Klebsiella pneumoniae carbapenemase (KPC-2). Antimicrob Agents Chemother. 2013 Jul; 57(7):3398-401.
Score: 0.087
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Probing beta-lactamase structure and function using random replacement mutagenesis. Proteins. 1992 Sep; 14(1):29-44.
Score: 0.084
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Identification of amino acid substitutions that alter the substrate specificity of TEM-1 beta-lactamase. J Bacteriol. 1992 Aug; 174(16):5237-43.
Score: 0.083
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Identification of a ?-lactamase inhibitory protein variant that is a potent inhibitor of Staphylococcus PC1 ?-lactamase. J Mol Biol. 2011 Mar 11; 406(5):730-44.
Score: 0.075
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Identification and characterization of beta-lactamase inhibitor protein-II (BLIP-II) interactions with beta-lactamases using phage display. Protein Eng Des Sel. 2010 Jun; 23(6):469-78.
Score: 0.071
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Fine mapping of the sequence requirements for binding of beta-lactamase inhibitory protein (BLIP) to TEM-1 beta-lactamase using a genetic screen for BLIP function. J Mol Biol. 2009 Jun 05; 389(2):401-12.
Score: 0.066
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Structural insight into the kinetics and DeltaCp of interactions between TEM-1 beta-lactamase and beta-lactamase inhibitory protein (BLIP). J Biol Chem. 2009 Jan 02; 284(1):595-609.
Score: 0.064
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Thermodynamic investigation of the role of contact residues of beta-lactamase-inhibitory protein for binding to TEM-1 beta-lactamase. J Biol Chem. 2007 Jun 15; 282(24):17676-84.
Score: 0.058
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Experimental evolution of gene duplicates in a bacterial plasmid model. J Mol Evol. 2007 Feb; 64(2):215-22.
Score: 0.057
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Copy number flexibility facilitates heteroresistance to increasing antibiotic pressure and threatens the beta-lactam pipeline. Nat Commun. 2025 Jul 01; 16(1):5721.
Score: 0.051
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The D-methyl group in beta-lactamase evolution: evidence from the Y221G and GC1 mutants of the class C beta-lactamase of Enterobacter cloacae P99. Biochemistry. 2005 May 24; 44(20):7543-52.
Score: 0.051
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Chromophoric spin-labeled beta-lactam antibiotics for ENDOR structural characterization of reaction intermediates of class A and class C beta-lactamases. Spectrochim Acta A Mol Biomol Spectrosc. 2004 May; 60(6):1279-89.
Score: 0.047
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A broad-spectrum peptide inhibitor of beta-lactamase identified using phage display and peptide arrays. Protein Eng. 2003 Nov; 16(11):853-60.
Score: 0.045
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An analysis of why highly similar enzymes evolve differently. Genetics. 2003 Feb; 163(2):457-66.
Score: 0.043
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Unveiling the structural features that regulate carbapenem deacylation in KPC-2 through QM/MM and interpretable machine learning. Phys Chem Chem Phys. 2023 Jan 04; 25(2):1349-1362.
Score: 0.043
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Slow Protein Dynamics Elicits New Enzymatic Functions by Means of Epistatic Interactions. Mol Biol Evol. 2022 10 07; 39(10).
Score: 0.042
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Binding properties of a peptide derived from beta-lactamase inhibitory protein. Antimicrob Agents Chemother. 2001 Dec; 45(12):3279-86.
Score: 0.040
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Protein minimization by random fragmentation and selection. Protein Eng. 2001 Jul; 14(7):487-92.
Score: 0.039
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A secondary drug resistance mutation of TEM-1 beta-lactamase that suppresses misfolding and aggregation. Proc Natl Acad Sci U S A. 2001 Jan 02; 98(1):283-8.
Score: 0.037
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Use of the arabinose p(bad) promoter for tightly regulated display of proteins on bacteriophage. Gene. 2000 Jun 27; 251(2):187-97.
Score: 0.036
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Structure-function analysis of alpha-helix H4 using PSE-4 as a model enzyme representative of class A beta-lactamases. Protein Eng. 2000 Apr; 13(4):267-74.
Score: 0.035
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Contributions of aspartate 49 and phenylalanine 142 residues of a tight binding inhibitory protein of beta-lactamases. J Biol Chem. 1999 Jan 22; 274(4):2394-400.
Score: 0.033
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Display of functional beta-lactamase inhibitory protein on the surface of M13 bacteriophage. Antimicrob Agents Chemother. 1998 Nov; 42(11):2893-7.
Score: 0.032
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Roles of amino acids 161 to 179 in the PSE-4 omega loop in substrate specificity and in resistance to ceftazidime. Antimicrob Agents Chemother. 1998 Oct; 42(10):2576-83.
Score: 0.032
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The rate-limiting step in the folding of the cis-Pro167Thr mutant of TEM-1 beta-lactamase is the trans to cis isomerization of a non-proline peptide bond. Proteins. 1996 May; 25(1):104-11.
Score: 0.027
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Outbreak of ceftazidime resistance due to a novel extended-spectrum beta-lactamase in isolates from cancer patients. Antimicrob Agents Chemother. 1992 Sep; 36(9):1991-6.
Score: 0.021
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2-Substituted 4,5-dihydrothiazole-4-carboxylic acids are novel inhibitors of metallo-?-lactamases. Bioorg Med Chem Lett. 2012 Oct 01; 22(19):6229-32.
Score: 0.021
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Penicillin-derived inhibitors that simultaneously target both metallo- and serine-beta-lactamases. Bioorg Med Chem Lett. 2004 Mar 08; 14(5):1299-304.
Score: 0.012
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Characterization of a PSE-4 mutant with different properties in relation to penicillanic acid sulfones: importance of residues 216 to 218 in class A beta-lactamases. Antimicrob Agents Chemother. 1998 Sep; 42(9):2319-25.
Score: 0.008