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VLADIMIR DIDENKO

TitleAssociate Professor
InstitutionBaylor College of Medicine
DepartmentDepartment of Neurosurgery
Address2002 HOLCOMBE
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    Other Positions
    TitleAssociate Professor
    InstitutionBaylor College of Medicine
    DepartmentDepartment of Molecular & Cellular Biology
    DivisionMolecular & Cellular Biology


    Collapse Research 
    Collapse research activities and funding
    R01GM148812     (DIDENKO, VLADIMIR V)Sep 21, 2022 - Jul 31, 2026
    NIH
    FRET detection and in situ quantification of efferocytosis using designed enzymatic activity
    Role: Principal Investigator
    R21AG071978     (DIDENKO, VLADIMIR V)Sep 30, 2021 - May 31, 2024
    NIH
    In situ detection of stalled cleavage complexes for studies in aging
    Role: Principal Investigator
    R21CA255979     (DIDENKO, VLADIMIR V)Sep 10, 2021 - Aug 31, 2024
    NIH
    In situ assay imaging nuclear RNA exosome activity for cancer studies
    Role: Principal Investigator
    R21AG073887     (DIDENKO, VLADIMIR V)Aug 15, 2021 - Jul 31, 2023
    NIH
    FRET assay for in situ assessment of nucleolytic lysosomal cell death in Alzheimer's neurodegeneration
    Role: Principal Investigator
    R21NS101620     (DIDENKO, VLADIMIR V)Mar 15, 2017 - Feb 29, 2020
    NIH
    Imaging mRNA turnover in P-bodies and its application to brain ischemia
    Role: Principal Investigator
    R21EB022788     (DIDENKO, VLADIMIR V)Mar 1, 2017 - Dec 31, 2019
    NIH
    Express assay for specific fluorescence imaging of apoptosis via phosphatase-assisted topoligation.
    Role: Principal Investigator
    R21AR066931     (DIDENKO, VLADIMIR V)Jul 1, 2014 - May 31, 2017
    NIH
    APMV-topoisomerase assay for comprehensive assessment of efferocytosis in tissue
    Role: Principal Investigator
    R01NS082553     (DIDENKO, VLADIMIR V)Apr 1, 2014 - Mar 31, 2018
    NIH
    FRET probes for analysis of serpin-mediated apoptosis in focal brain ischemia
    Role: Principal Investigator
    R21CA178365     (DIDENKO, VLADIMIR V)Jul 1, 2013 - Jun 30, 2016
    NIH
    Apoptosis driven by elastase inhibitor: new approach to detection and study.
    Role: Principal Investigator
    R21NS064403     (DIDENKO, VLADIMIR V)Jun 15, 2009 - May 31, 2012
    NIH
    Assay for dual detection of apoptosis
    Role: Principal Investigator
    R01NS062842     (DIDENKO, VLADIMIR V)Apr 1, 2009 - Mar 31, 2014
    NIH
    Ligatable fluorescent probes using energy transfer for apoptosis detection
    Role: Principal Investigator
    R21EB006301     (DIDENKO, VLADIMIR V)Apr 1, 2006 - Mar 31, 2010
    NIH
    Semi-artificial nanomachines for detection of DNA damage and apoptosis
    Role: Principal Investigator
    R21NS054855     (DIDENKO, VLADIMIR V)Mar 29, 2006 - Feb 28, 2009
    NIH
    Detection of Topoisomerase II Mediated DNA Damage in Stroke
    Role: Principal Investigator
    R03AG022664     (DIDENKO, VLADIMIR V)Sep 30, 2003 - Aug 31, 2006
    NIH
    Free radicals detection in aging brain by fullerenes
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Minchew CL, Didenko VV. Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer. J Vis Exp. 2025 Jul 25; (221). PMID: 40788859.
      Citations:    Fields:    Translation:HumansCells
    2. Minchew CL, Didenko VV. Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D. Methods Mol Biol. 2017; 1554:229-236. PMID: 28185196; PMCID: PMC5654642.
      Citations:    Fields:    Translation:Cells
    3. Hauser P, Wang S, Didenko VV. Apoptotic Bodies: Selective Detection in Extracellular Vesicles. Methods Mol Biol. 2017; 1554:193-200. PMID: 28185192.
      Citations: 42     Fields:    Translation:Cells
    4. Didenko VV. Express FRET Labeling and Analysis of Phagocytic Clearance. Methods Mol Biol. 2017; 1644:3-11. PMID: 28710749; PMCID: PMC5654643.
      Citations:    Fields:    Translation:HumansCells
    5. Minchew CL, Didenko VV. Quick Detection of DNase II-Type Breaks in Formalin-Fixed Tissue Sections. Methods Mol Biol. 2017; 1644:113-119. PMID: 28710757; PMCID: PMC5654645.
      Citations: 2     Fields:    Translation:HumansAnimalsCells
    6. Didenko VV. Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation. Methods Mol Biol. 2017; 1644:167-177. PMID: 28710763; PMCID: PMC5673075.
      Citations:    Fields:    Translation:Humans
    7. Minchew CL, Didenko VV. Nanoblinker: Brownian motion powered bio-nanomachine for FRET detection of phagocytic phase of apoptosis. PLoS One. 2014; 9(9):e108734. PMID: 25268504; PMCID: PMC4182547.
      Citations: 3     Fields:    Translation:HumansAnimalsCells
    8. Minchew CL, Didenko VV. Assessing phagocytic clearance of cell death in experimental stroke by ligatable fluorescent probes. J Vis Exp. 2014 May 27; (87). PMID: 24894100; PMCID: PMC4207364.
      Citations: 4     Fields:    Translation:Animals
    9. Didenko VV. Selective detection of phagocytic phase of apoptosis in fixed tissue sections. Methods Mol Biol. 2014; 1094:159-66. PMID: 24162987.
      Citations: 1     Fields:    Translation:HumansCells
    10. Minchew CL, Didenko VV. Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies. Methods Mol Biol. 2014; 1094:167-75. PMID: 24162988.
      Citations:    Fields:    Translation:HumansCells
    11. Minchew CL, Didenko VV. In vitro assembly of semi-artificial molecular machine and its use for detection of DNA damage. J Vis Exp. 2012 Jan 11; (59):e3628. PMID: 22258163; PMCID: PMC3353514.
      Citations: 3     Fields:    
    12. Minchew CL, Didenko VV. Fluorescent probes detecting the phagocytic phase of apoptosis: enzyme-substrate complexes of topoisomerase and DNA. Molecules. 2011 Jun 03; 16(6):4599-614. PMID: 21642935; PMCID: PMC3324561.
      Citations: 10     Fields:    Translation:HumansCells
    13. Didenko VV. 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach. Methods Mol Biol. 2011; 682:77-87. PMID: 21057922; PMCID: PMC3324587.
      Citations: 8     Fields:    Translation:AnimalsCells
    14. Hornsby PJ, Didenko VV. In situ ligation: a decade and a half of experience. Methods Mol Biol. 2011; 682:49-63. PMID: 21057920; PMCID: PMC3324560.
      Citations: 12     Fields:    Translation:Animals
    15. Didenko VV. In situ ligation simplified: using PCR fragments for detection of double-strand DNA breaks in tissue sections. Methods Mol Biol. 2011; 682:65-75. PMID: 21057921; PMCID: PMC3324586.
      Citations: 3     Fields:    Translation:Animals
    16. Didenko VV. In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase. Methods Mol Biol. 2011; 682:37-48. PMID: 21057919; PMCID: PMC3324588.
      Citations: 3     Fields:    Translation:Animals
    17. Didenko VV, Baskin DS. Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots. Biotechniques. 2006 Mar; 40(3):295-6, 298, 300-2. PMID: 16568818; PMCID: PMC1855219.
      Citations:    Fields:    
    18. Didenko VV. Oscillating probe for dual detection of 5'PO4 and 5'OH DNA breaks in tissue sections. Methods Mol Biol. 2006; 335:59-69. PMID: 16785620.
      Citations: 3     Fields:    Translation:Cells
    19. Didenko VV, Ngo H, Baskin DS. Polyethyleneimine as a transmembrane carrier of fluorescently labeled proteins and antibodies. Anal Biochem. 2005 Sep 15; 344(2):168-73. PMID: 16095551; PMCID: PMC1853266.
      Citations:    
    20. Didenko VV, Moore VC, Baskin DS, Smalley RE. Visualization of individual single-walled carbon nanotubes by fluorescent polymer wrapping. Nano Lett. 2005 Aug; 5(8):1563-7. PMID: 16089489; PMCID: PMC1853265.
      Citations: 7     Fields:    
    21. Didenko VV, Minchew CL, Shuman S, Baskin DS. Semi-artificial Fluorescent Molecular Machine for DNA Damage Detection. Nano Lett. 2004 Dec; 4(12):2461-6. PMID: 17330146; PMCID: PMC1805678.
      Citations: 8     Fields:    
    22. Baskin DS, Ngo H, Didenko VV. Thimerosal induces DNA breaks, caspase-3 activation, membrane damage, and cell death in cultured human neurons and fibroblasts. Toxicol Sci. 2003 Aug; 74(2):361-8. PMID: 12773768; PMCID: PMC1892749.
      Citations: 21     Fields:    Translation:HumansCells
    23. Didenko VV, Ngo H, Baskin DS. Early necrotic DNA degradation: presence of blunt-ended DNA breaks, 3' and 5' overhangs in apoptosis, but only 5' overhangs in early necrosis. Am J Pathol. 2003 May; 162(5):1571-8. PMID: 12707041; PMCID: PMC1851179.
      Citations: 29     Fields:    Translation:HumansCells
    24. Didenko VV, Ngo H, Minchew CL, Boudreaux DJ, Widmayer MA, Baskin DS. Visualization of irreparable ischemic damage in brain by selective labeling of double strand blunt-ended DNA breaks. Mol Med. 2002 Dec; 8(12):818-23. PMID: 12606816; PMCID: PMC2039967.
      Citations: 4     Fields:    Translation:Animals
    25. Didenko VV, Ngo H, Minchew CL, Boudreaux DJ, Widmayer MA, Baskin DS. Caspase-3-dependent and -independent apoptosis in focal brain ischemia. Mol Med. 2002 Jul; 8(7):347-52. PMID: 12393932; PMCID: PMC2040004.
      Citations: 13     Fields:    Translation:AnimalsCells
    26. Didenko VV, Ngo HN, Minchew C, Baskin DS. Apoptosis of T lymphocytes invading glioblastomas multiforme: a possible tumor defense mechanism. J Neurosurg. 2002 Mar; 96(3):580-4. PMID: 11883844; PMCID: PMC1853267.
      Citations: 36     Fields:    Translation:HumansCells
    27. Didenko VV. Early shape-shifting FRET probe. Biotechniques. 2002 Feb; 32(2):272. PMID: 11848401.
      Citations:    Fields:    Translation:Cells
    28. Hornsby PJ, Didenko VV. In situ DNA ligation as a method for labeling apoptotic cells in tissue sections. An overview. Methods Mol Biol. 2002; 203:133-41. PMID: 12073437.
      Citations: 4     Fields:    Translation:AnimalsCells
    29. Didenko VV, Ngo H, Baskin DS. In situ detection of double-strand DNA breaks with terminal 5'OH groups. Methods Mol Biol. 2002; 203:153-9. PMID: 12073439.
      Citations: 3     Fields:    Translation:Animals
    30. Didenko VV. Detection of specific double-strand DNA breaks and apoptosis in situ using T4 DNA ligase. Methods Mol Biol. 2002; 203:143-51. PMID: 12073438.
      Citations: 9     Fields:    
    31. Didenko VV. DNA probes using fluorescence resonance energy transfer (FRET): designs and applications. Biotechniques. 2001 Nov; 31(5):1106-16, 1118, 1120-1. PMID: 11730017; PMCID: PMC1941713.
      Citations: 83     Fields:    Translation:HumansAnimalsCells
    32. Ding B, Price RL, Goldsmith EC, Borg TK, Yan X, Douglas PS, Weinberg EO, Bartunek J, Thielen T, Didenko VV, Lorell BH. Left ventricular hypertrophy in ascending aortic stenosis mice: anoikis and the progression to early failure. Circulation. 2000 Jun 20; 101(24):2854-62. PMID: 10859293.
      Citations: 36     Fields:    Translation:Animals
    33. Bales ES, Dietrich C, Bandyopadhyay D, Schwahn DJ, Xu W, Didenko V, Leiss P, Conrad N, Pereira-Smith O, Orengo I, Medrano EE. High levels of expression of p27KIP1 and cyclin E in invasive primary malignant melanomas. J Invest Dermatol. 1999 Dec; 113(6):1039-46. PMID: 10594749.
      Citations: 15     Fields:    Translation:Humans
    34. Didenko VV, Boudreaux DJ, Baskin DS. Substantial background reduction in ligase-based apoptosis detection using newly designed hairpin oligonucleotide probes. Biotechniques. 1999 Dec; 27(6):1130-2. PMID: 10631490; PMCID: PMC1892751.
      Citations: 9     Fields:    Translation:AnimalsCells
    35. Didenko VV, Wang X, Yang L, Hornsby PJ. DNA damage and p21(WAF1/CIP1/SDI1) in experimental injury of the rat adrenal cortex and trauma-associated damage of the human adrenal cortex. J Pathol. 1999 Sep; 189(1):119-26. PMID: 10451498.
      Citations: 2     Fields:    Translation:HumansAnimals
    36. Bérubé NG, Swanson XH, Bertram MJ, Kittle JD, Didenko V, Baskin DS, Smith JR, Pereira-Smith OM, B?rub? NG. Cloning and characterization of CRF, a novel C1q-related factor, expressed in areas of the brain involved in motor function. Brain Res Mol Brain Res. 1999 Jan 08; 63(2):233-40. PMID: 9878755.
      Citations: 15     Fields:    Translation:HumansAnimalsCells
    37. Bozkurt B, Kribbs SB, Clubb FJ, Michael LH, Didenko VV, Hornsby PJ, Seta Y, Oral H, Spinale FG, Mann DL. Pathophysiologically relevant concentrations of tumor necrosis factor-alpha promote progressive left ventricular dysfunction and remodeling in rats. Circulation. 1998 Apr 14; 97(14):1382-91. PMID: 9577950.
      Citations: 213     Fields:    Translation:AnimalsCells
    38. Didenko VV, Tunstead JR, Hornsby PJ. Biotin-labeled hairpin oligonucleotides: probes to detect double-strand breaks in DNA in apoptotic cells. Am J Pathol. 1998 Apr; 152(4):897-902. PMID: 9546350; PMCID: PMC1858232.
      Citations: 13     Fields:    Translation:HumansAnimalsCells
    39. Didenko VV, Hornsby PJ. Presence of double-strand breaks with single-base 3' overhangs in cells undergoing apoptosis but not necrosis. J Cell Biol. 1996 Dec; 135(5):1369-76. PMID: 8947557; PMCID: PMC2121088.
      Citations: 40     Fields:    Translation:HumansAnimals
    40. Didenko VV, Hornsby PJ. A quantitative luminescence assay for nonradioactive nucleic acid probes. J Histochem Cytochem. 1996 Jun; 44(6):657-60. PMID: 8666750.
      Citations: 1     Fields:    Translation:Animals
    41. Didenko VV, Wang X, Yang L, Hornsby PJ. Expression of p21(WAF1/CIP1/SDI1) and p53 in apoptotic cells in the adrenal cortex and induction by ischemia/reperfusion injury. J Clin Invest. 1996 Apr 01; 97(7):1723-31. PMID: 8601638; PMCID: PMC507237.
      Citations: 12     Fields:    Translation:HumansAnimalsCells
    42. Yang L, Didenko VV, Noda A, Bilyeu TA, Darlington DJ, Smith JR, Hornsby PJ. Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture. Exp Cell Res. 1995 Nov; 221(1):126-31. PMID: 7589237.
      Citations: 4     Fields:    Translation:HumansAnimalsCells
    43. Didenko VV. Biotinylation of DNA on membrane supports: a procedure for preparation and easy control of labeling of nonradioactive single-stranded nucleic acid probes. Anal Biochem. 1993 Aug 15; 213(1):75-8. PMID: 8238885.
      Citations:    Fields:    Translation:Cells
    44. Didenko VV. Polyethylene tube: an alternative support for synthesis and purification of biotin-labeled single-stranded DNA probes. Nucleic Acids Res. 1993 May 11; 21(9):2279-80. PMID: 8502583; PMCID: PMC309513.
      Citations:    Fields:    Translation:Cells
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