Polymerase Chain Reaction
"Polymerase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Descriptor ID |
D016133
|
MeSH Number(s) |
E05.393.620.500
|
Concept/Terms |
Polymerase Chain Reaction- Polymerase Chain Reaction
- Polymerase Chain Reactions
- Reaction, Polymerase Chain
- Reactions, Polymerase Chain
- PCR
Inverse PCR- Inverse PCR
- PCR, Inverse
- Inverse Polymerase Chain Reaction
Anchored PCR- Anchored PCR
- PCR, Anchored
- Anchored Polymerase Chain Reaction
|
Below are MeSH descriptors whose meaning is more general than "Polymerase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Polymerase Chain Reaction".
This graph shows the total number of publications written about "Polymerase Chain Reaction" by people in this website by year, and whether "Polymerase Chain Reaction" was a major or minor topic of these publications.
To see the data from this visualization as text,
click here.
Year | Major Topic | Minor Topic | Total |
---|
1994 | 14 | 87 | 101 |
1995 | 12 | 113 | 125 |
1996 | 26 | 101 | 127 |
1997 | 12 | 112 | 124 |
1998 | 11 | 99 | 110 |
1999 | 8 | 65 | 73 |
2000 | 10 | 86 | 96 |
2001 | 4 | 74 | 78 |
2002 | 12 | 91 | 103 |
2003 | 7 | 103 | 110 |
2004 | 5 | 80 | 85 |
2005 | 7 | 87 | 94 |
2006 | 4 | 80 | 84 |
2007 | 6 | 73 | 79 |
2008 | 7 | 80 | 87 |
2009 | 4 | 65 | 69 |
2010 | 5 | 100 | 105 |
2011 | 10 | 76 | 86 |
2012 | 4 | 47 | 51 |
2013 | 7 | 41 | 48 |
2014 | 4 | 33 | 37 |
2015 | 3 | 43 | 46 |
2016 | 7 | 34 | 41 |
2017 | 4 | 21 | 25 |
2018 | 2 | 14 | 16 |
2019 | 3 | 12 | 15 |
2020 | 1 | 9 | 10 |
2021 | 0 | 9 | 9 |
2022 | 1 | 2 | 3 |
2023 | 0 | 3 | 3 |
2024 | 3 | 2 | 5 |
To return to the timeline,
click here.
Below are the most recent publications written about "Polymerase Chain Reaction" by people in Profiles.
-
Assessing the Diagnostic Performance and Clinical Utility of Nasal Methicillin-Resistant Staphylococcus aureus PCR Testing in Pediatric Orbital Cellulitis. J Pediatric Infect Dis Soc. 2024 Aug 24; 13(8):430-433.
-
Evaluation of the presence of integrons, sul and smqnr genes and the prevalence of antibiotic resistance in Stenotrophomonas maltophilia clinical isolates. Indian J Med Microbiol. 2024 May-Jun; 49:100612.
-
Validation of Single-Nucleotide Mosaic Variants Through Droplet Digital PCR. Curr Protoc. 2024 May; 4(5):e1041.
-
Sex Genotyping Mice by Polymerase Chain Reaction. Cold Spring Harb Protoc. 2024 01 02; 2024(1):108062.
-
Isolation of XO Subclones from XY Murine Embryonic Stem Cells. Cold Spring Harb Protoc. 2024 01 02; 2024(1):108038.
-
GetPrimers: A generalized PCR-based genetic targeting primer designer enabling easy and standardized targeted gene modification across multiple systems. Yeast. 2024 Jan; 41(1-2):19-34.
-
Undetectable JC virus CSF PCR in patients with JC virus-induced progressive multifocal leukoencephalopathy. J Neurovirol. 2023 02; 29(1):94-99.
-
DETECTION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS 1A IN ARCHIVAL TISSUE USING RNASCOPE? IN SITU HYBRIDIZATION. J Zoo Wildl Med. 2023 Jan; 53(4):661-669.
-
Pyrococcus furiosus Argonaute Combined with Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Enterocytozoon hepatopenaei. J Agric Food Chem. 2023 Jan 11; 71(1):944-951.
-
Highly sensitive genotyping of MTHFR C677T polymorphisms using a novel RPA-LDR-qPCR assay. Acta Biochim Biophys Sin (Shanghai). 2022 10 25; 54(11):1753-1756.