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HIRAM F. GILBERT

TitleProfessor Emeritus
InstitutionBaylor College of Medicine
DepartmentDepartment of Biochemistry & Molecular Biology
DivisionBiochemistry & Molecular Biology
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    R01GM025347     (GILBERT, HIRAM F)Dec 1, 1978 - Nov 30, 1987
    NIH/NIGMS
    ENZYME CATALYSIS OF PROTON TRANSFER FROM CARBON
    Role: Principal Investigator

    R01HL028521     (GILBERT, HIRAM F)Apr 1, 1982 - Jun 30, 1995
    NIH/NHLBI
    REDOX REGULATION OF HMG COA REDUCTASE
    Role: Principal Investigator

    K04HL001020     (GILBERT, HIRAM F)Jul 1, 1982 - Jun 30, 1987
    NIH/NHLBI
    REDOX CONTROL OF HMG-COA REDUCTASE ACTIVITY
    Role: Principal Investigator

    R01GM040379     (GILBERT, HIRAM F)Jul 1, 1988 - Sep 14, 1995
    NIH/NIGMS
    Catalysis of Thiol-Disulfide Exchange
    Role: Principal Investigator

    K12GM084897     (SLAUGHTER, GAYLE)Aug 1, 2008 - Jul 31, 2017
    NIH/NIGMS
    Research Education and Career Horizons Program
    Role: Co-Principal Investigator

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Stojanoski V, Chow DC, Hu L, Sankaran B, Gilbert HF, Prasad BV, Palzkill T. A triple mutant in the O-loop of TEM-1 ß-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis. J Biol Chem. 2015 Apr 17; 290(16):10382-94. PMID: 25713062.
      View in: PubMed
    2. Adamski CJ, Cardenas AM, Brown NG, Horton LB, Sankaran B, Prasad BV, Gilbert HF, Palzkill T. Molecular basis for the catalytic specificity of the CTX-M extended-spectrum ß-lactamases. Biochemistry. 2015 Jan 20; 54(2):447-57. PMID: 25489790.
      View in: PubMed
    3. Yuan J, Cardenas AM, Gilbert HF, Palzkill T. Determination of the amino acid sequence requirements for catalysis by the highly proficient orotidine monophosphate decarboxylase. Protein Sci. 2011 Nov; 20(11):1891-906. PMID: 21898650.
      View in: PubMed
    4. Arredondo SA, Chen TF, Riggs AF, Gilbert HF, Georgiou G. Role of dimerization in the catalytic properties of the Escherichia coli disulfide isomerase DsbC. J Biol Chem. 2009 Sep 04; 284(36):23972-9. PMID: 19581640.
      View in: PubMed
    5. Kim JH, Zhao Y, Pan X, He X, Gilbert HF. The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization. J Biol Chem. 2009 Apr 17; 284(16):10400-8. PMID: 19233841.
      View in: PubMed
    6. Arredondo S, Segatori L, Gilbert HF, Georgiou G. De novo design and evolution of artificial disulfide isomerase enzymes analogous to the bacterial DsbC. J Biol Chem. 2008 Nov 14; 283(46):31469-76. PMID: 18782764.
      View in: PubMed
    7. Segatori L, Murphy L, Arredondo S, Kadokura H, Gilbert H, Beckwith J, Georgiou G. Conserved role of the linker alpha-helix of the bacterial disulfide isomerase DsbC in the avoidance of misoxidation by DsbB. J Biol Chem. 2006 Feb 24; 281(8):4911-9. PMID: 16280324.
      View in: PubMed
    8. Samudio I, Konopleva M, Hail N, Shi YX, McQueen T, Hsu T, Evans R, Honda T, Gribble GW, Sporn M, Gilbert HF, Safe S, Andreeff M. 2-Cyano-3,12-dioxooleana-1,9-dien-28-imidazolide (CDDO-Im) directly targets mitochondrial glutathione to induce apoptosis in pancreatic cancer. J Biol Chem. 2005 Oct 28; 280(43):36273-82. PMID: 16118208.
      View in: PubMed
    9. Xiao R, Lundström-Ljung J, Holmgren A, Gilbert HF. Catalysis of thiol/disulfide exchange. Glutaredoxin 1 and protein-disulfide isomerase use different mechanisms to enhance oxidase and reductase activities. J Biol Chem. 2005 Jun 03; 280(22):21099-106. PMID: 15814611.
      View in: PubMed
    10. Wilkinson B, Xiao R, Gilbert HF. A structural disulfide of yeast protein-disulfide isomerase destabilizes the active site disulfide of the N-terminal thioredoxin domain. J Biol Chem. 2005 Mar 25; 280(12):11483-7. PMID: 15649885.
      View in: PubMed
    11. Xiao R, Wilkinson B, Solovyov A, Winther JR, Holmgren A, Lundström-Ljung J, Gilbert HF. The contributions of protein disulfide isomerase and its homologues to oxidative protein folding in the yeast endoplasmic reticulum. J Biol Chem. 2004 Nov 26; 279(48):49780-6. PMID: 15377672.
      View in: PubMed
    12. Segatori L, Paukstelis PJ, Gilbert HF, Georgiou G. Engineered DsbC chimeras catalyze both protein oxidation and disulfide-bond isomerization in Escherichia coli: Reconciling two competing pathways. Proc Natl Acad Sci U S A. 2004 Jul 06; 101(27):10018-23. PMID: 15220477.
      View in: PubMed
    13. Solovyov A, Xiao R, Gilbert HF. Sulfhydryl oxidation, not disulfide isomerization, is the principal function of protein disulfide isomerase in yeast Saccharomyces cerevisiae. J Biol Chem. 2004 Aug 13; 279(33):34095-100. PMID: 15175335.
      View in: PubMed
    14. Wilkinson B, Gilbert HF. Protein disulfide isomerase. Biochim Biophys Acta. 2004 Jun 01; 1699(1-2):35-44. PMID: 15158710.
      View in: PubMed
    15. Solovyov A, Gilbert HF. Zinc-dependent dimerization of the folding catalyst, protein disulfide isomerase. Protein Sci. 2004 Jul; 13(7):1902-7. PMID: 15169950.
      View in: PubMed
    16. Schwaller M, Wilkinson B, Gilbert HF. Reduction-reoxidation cycles contribute to catalysis of disulfide isomerization by protein-disulfide isomerase. J Biol Chem. 2003 Feb 28; 278(9):7154-9. PMID: 12486139.
      View in: PubMed
    17. Liu CW, Millen L, Roman TB, Xiong H, Gilbert HF, Noiva R, DeMartino GN, Thomas PJ. Conformational remodeling of proteasomal substrates by PA700, the 19 S regulatory complex of the 26 S proteasome. J Biol Chem. 2002 Jul 26; 277(30):26815-20. PMID: 12011044.
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    18. Xiao R, Solovyov A, Gilbert HF, Holmgren A, Lundström-Ljung J. Combinations of protein-disulfide isomerase domains show that there is little correlation between isomerase activity and wild-type growth. J Biol Chem. 2001 Jul 27; 276(30):27975-80. PMID: 11375405.
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    19. Zheng J, Gilbert HF. Discrimination between native and non-native disulfides by protein-disulfide isomerase. J Biol Chem. 2001 May 11; 276(19):15747-52. PMID: 11278905.
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    20. Primm TP, Gilbert HF. Hormone binding by protein disulfide isomerase, a high capacity hormone reservoir of the endoplasmic reticulum. J Biol Chem. 2001 Jan 05; 276(1):281-6. PMID: 11035025.
      View in: PubMed
    21. Sideraki V, Huang W, Palzkill T, Gilbert HF. A secondary drug resistance mutation of TEM-1 beta-lactamase that suppresses misfolding and aggregation. Proc Natl Acad Sci U S A. 2001 Jan 02; 98(1):283-8. PMID: 11114163.
      View in: PubMed
    22. Sideraki V, Gilbert HF. Mechanism of the antichaperone activity of protein disulfide isomerase: facilitated assembly of large, insoluble aggregates of denatured lysozyme and PDI. Biochemistry. 2000 Feb 08; 39(5):1180-8. PMID: 10653666.
      View in: PubMed
    23. Zhan X, Schwaller M, Gilbert HF, Georgiou G. Facilitating the formation of disulfide bonds in the Escherichia coli periplasm via coexpression of yeast protein disulfide isomerase. Biotechnol Prog. 1999 Nov-Dec; 15(6):1033-8. PMID: 10585186.
      View in: PubMed
    24. Hoober KL, Sheasley SL, Gilbert HF, Thorpe C. Sulfhydryl oxidase from egg white. A facile catalyst for disulfide bond formation in proteins and peptides. J Biol Chem. 1999 Aug 06; 274(32):22147-50. PMID: 10428777.
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    25. Bessette PH, Cotto JJ, Gilbert HF, Georgiou G. In vivo and in vitro function of the Escherichia coli periplasmic cysteine oxidoreductase DsbG. J Biol Chem. 1999 Mar 19; 274(12):7784-92. PMID: 10075670.
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    26. Gilbert HF. Protein disulfide isomerase. Methods Enzymol. 1998; 290:26-50. PMID: 9534149.
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    27. Puig A, Primm TP, Surendran R, Lee JC, Ballard KD, Orkiszewski RS, Makarov V, Gilbert HF. A 21-kDa C-terminal fragment of protein-disulfide isomerase has isomerase, chaperone, and anti-chaperone activities. J Biol Chem. 1997 Dec 26; 272(52):32988-94. PMID: 9407079.
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    28. Gilbert HF. Protein disulfide isomerase and assisted protein folding. J Biol Chem. 1997 Nov 21; 272(47):29399-402. PMID: 9367991.
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    29. Walker KW, Gilbert HF. Scanning and escape during protein-disulfide isomerase-assisted protein folding. J Biol Chem. 1997 Apr 04; 272(14):8845-8. PMID: 9082998.
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    30. Primm TP, Walker KW, Gilbert HF. Facilitated protein aggregation. Effects of calcium on the chaperone and anti-chaperone activity of protein disulfide-isomerase. J Biol Chem. 1996 Dec 27; 271(52):33664-9. PMID: 8969236.
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    31. Chang Z, Primm TP, Jakana J, Lee IH, Serysheva I, Chiu W, Gilbert HF, Quiocho FA. Mycobacterium tuberculosis 16-kDa antigen (Hsp16.3) functions as an oligomeric structure in vitro to suppress thermal aggregation. J Biol Chem. 1996 Mar 22; 271(12):7218-23. PMID: 8636160.
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    32. Walker KW, Lyles MM, Gilbert HF. Catalysis of oxidative protein folding by mutants of protein disulfide isomerase with a single active-site cysteine. Biochemistry. 1996 Feb 13; 35(6):1972-80. PMID: 8639681.
      View in: PubMed
    33. Walker KW, Gilbert HF. Oxidation of kinetically trapped thiols by protein disulfide isomerase. Biochemistry. 1995 Oct 17; 34(41):13642-50. PMID: 7577954.
      View in: PubMed
    34. Gilbert HF. Thiol/disulfide exchange equilibria and disulfide bond stability. Methods Enzymol. 1995; 251:8-28. PMID: 7651233.
      View in: PubMed
    35. Lyles MM, Gilbert HF. Mutations in the thioredoxin sites of protein disulfide isomerase reveal functional nonequivalence of the N- and C-terminal domains. J Biol Chem. 1994 Dec 09; 269(49):30946-52. PMID: 7983029.
      View in: PubMed
    36. Walker KW, Gilbert HF. Effect of redox environment on the in vitro and in vivo folding of RTEM-1 beta-lactamase and Escherichia coli alkaline phosphatase. J Biol Chem. 1994 Nov 11; 269(45):28487-93. PMID: 7961790.
      View in: PubMed
    37. Puig A, Gilbert HF. Anti-chaperone behavior of BiP during the protein disulfide isomerase-catalyzed refolding of reduced denatured lysozyme. J Biol Chem. 1994 Oct 14; 269(41):25889-96. PMID: 7929293.
      View in: PubMed
    38. Gilbert HF. Protein chaperones and protein folding. Curr Opin Biotechnol. 1994 Oct; 5(5):534-9. PMID: 7765469.
      View in: PubMed
    39. Puig A, Lyles MM, Noiva R, Gilbert HF. The role of the thiol/disulfide centers and peptide binding site in the chaperone and anti-chaperone activities of protein disulfide isomerase. J Biol Chem. 1994 Jul 22; 269(29):19128-35. PMID: 7913469.
      View in: PubMed
    40. Morjana NA, Gilbert HF. Catalysis of protein folding by agarose-immobilized protein disulfide isomerase. Protein Expr Purif. 1994 Apr; 5(2):144-8. PMID: 8054846.
      View in: PubMed
    41. Puig A, Gilbert HF. Protein disulfide isomerase exhibits chaperone and anti-chaperone activity in the oxidative refolding of lysozyme. J Biol Chem. 1994 Mar 11; 269(10):7764-71. PMID: 7907332.
      View in: PubMed
    42. Morjana NA, McKeone BJ, Gilbert HF. Guanidine hydrochloride stabilization of a partially unfolded intermediate during the reversible denaturation of protein disulfide isomerase. Proc Natl Acad Sci U S A. 1993 Mar 15; 90(6):2107-11. PMID: 8460117.
      View in: PubMed
    43. Cappel RE, Gilbert HF. Oxidative inactivation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase and subunit cross-linking involve different dithiol/disulfide centers. J Biol Chem. 1993 Jan 05; 268(1):342-8. PMID: 8416941.
      View in: PubMed
    44. Lu X, Gilbert HF, Harper JW. Conserved residues flanking the thiol/disulfide centers of protein disulfide isomerase are not essential for catalysis of thiol/disulfide exchange. Biochemistry. 1992 May 05; 31(17):4205-10. PMID: 1567868.
      View in: PubMed
    45. Reiners JJ, Kodari E, Cappel RE, Gilbert HF. Assessment of the antioxidant/prooxidant status of murine skin following topical treatment with 12-O-tetradecanoylphorbol-13-acetate and throughout the ontogeny of skin cancer. Part II: Quantitation of glutathione and glutathione disulfide. Carcinogenesis. 1991 Dec; 12(12):2345-52. PMID: 1747938.
      View in: PubMed
    46. Chung PM, Cappel RE, Gilbert HF. Inhibition of glutathione disulfide reductase by glutathione. Arch Biochem Biophys. 1991 Jul; 288(1):48-53. PMID: 1898023.
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    47. Morjana NA, Gilbert HF. Effect of protein and peptide inhibitors on the activity of protein disulfide isomerase. Biochemistry. 1991 May 21; 30(20):4985-90. PMID: 2036365.
      View in: PubMed
    48. Gilbert HF, Kruzel ML, Lyles MM, Harper JW. Expression and purification of recombinant rat protein disulfide isomerase from Escherichia coli. Protein Expr Purif. 1991 Apr-Jun; 2(2-3):194-8. PMID: 1821789.
      View in: PubMed
    49. Lyles MM, Gilbert HF. Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: pre-steady-state kinetics and the utilization of the oxidizing equivalents of the isomerase. Biochemistry. 1991 Jan 22; 30(3):619-25. PMID: 1988051.
      View in: PubMed
    50. Lyles MM, Gilbert HF. Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer. Biochemistry. 1991 Jan 22; 30(3):613-9. PMID: 1988050.
      View in: PubMed
    51. Cappel RE, Gilbert HF. The effects of NADPH and 3-hydroxy-3-methylglutaryl-CoA on the thiol/disulfide redox behavior of rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase. J Biol Chem. 1990 Sep 15; 265(26):15464-70. PMID: 2394734.
      View in: PubMed
    52. Gilbert HF. Molecular and cellular aspects of thiol-disulfide exchange. Adv Enzymol Relat Areas Mol Biol. 1990; 63:69-172. PMID: 2407068.
      View in: PubMed
    53. Gilbert HF. Catalysis of thiol/disulfide exchange: single-turnover reduction of protein disulfide-isomerase by glutathione and catalysis of peptide disulfide reduction. Biochemistry. 1989 Sep 05; 28(18):7298-305. PMID: 2819070.
      View in: PubMed
    54. Izbicka E, Gilbert HF. Examination of the role of thiolimidate formation in the cleavage of acetoacetyl-CoA catalyzed by thiolase I from porcine heart. Arch Biochem Biophys. 1989 Aug 01; 272(2):476-80. PMID: 2568819.
      View in: PubMed
    55. Cappel RE, Gilbert HF. The effects of mevinolin on the thiol/disulfide exchange between 3-hydroxy-3-methyglutaryl-coenzyme A reductase and glutathione. J Biol Chem. 1989 Jun 05; 264(16):9180-7. PMID: 2722823.
      View in: PubMed
    56. Cappel RE, Gilbert HF. Thiol/disulfide exchange between 3-hydroxy-3-methylglutaryl-CoA reductase and glutathione. A thermodynamically facile dithiol oxidation. J Biol Chem. 1988 Sep 05; 263(25):12204-12. PMID: 3410841.
      View in: PubMed
    57. Clancey CJ, Gilbert HF. Thiol/disulfide exchange in the thioredoxin-catalyzed reductive activation of spinach chloroplast fructose-1,6-bisphosphatase. Kinetics and thermodynamics. J Biol Chem. 1987 Oct 05; 262(28):13545-9. PMID: 2820974.
      View in: PubMed
    58. Walters DW, Gilbert HF. Thiol/disulfide exchange between rabbit muscle phosphofructokinase and glutathione. Kinetics and thermodynamics of enzyme oxidation. J Biol Chem. 1986 Nov 25; 261(33):15372-7. PMID: 2946673.
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    59. Cappel RE, Bremer JW, Timmons TM, Nelson TE, Gilbert HF. Thiol/disulfide redox equilibrium between glutathione and glycogen debranching enzyme (amylo-1,6-glucosidase/4-alpha-glucanotransferase) from rabbit muscle. J Biol Chem. 1986 Nov 25; 261(33):15385-9. PMID: 2946674.
      View in: PubMed
    60. Cappel RE, Gilbert HF. Cooperative behavior in the thiol oxidation of rabbit muscle glycogen phosphorylase in cysteamine/cystamine redox buffers. J Biol Chem. 1986 Nov 25; 261(33):15378-84. PMID: 3782073.
      View in: PubMed
    61. Walters DW, Gilbert HF. Thiol/disulfide redox equilibrium and kinetic behavior of chicken liver fatty acid synthase. J Biol Chem. 1986 Oct 05; 261(28):13135-43. PMID: 3759951.
      View in: PubMed
    62. Izbicka E, Gilbert HF. Fluorescence energy transfer measurements of spatial relationships between sulfhydryl groups of thiolase I from porcine heart. Biochemistry. 1984 Dec 18; 23(26):6383-8. PMID: 6152180.
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    63. Izbicka-Dimitrijevic E, Gilbert HF. Multiple oxidation products of sulfhydryl groups near the active site of thiolase I from porcine heart. Biochemistry. 1984 Sep 11; 23(19):4318-24. PMID: 6148962.
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    64. Gilbert HF. Redox control of enzyme activities by thiol/disulfide exchange. Methods Enzymol. 1984; 107:330-51. PMID: 6239077.
      View in: PubMed
    65. Luther MA, Gilbert HF, Lee JC. Self-association of rabbit muscle phosphofructokinase: role of subunit interaction in regulation of enzymatic activity. Biochemistry. 1983 Nov 22; 22(24):5494-500. PMID: 6228252.
      View in: PubMed
    66. Izbicka-Dimitrijevic E, Gilbert HF. Two sulfhydryl groups near the active site of thiolase I from porcine heart: modification of thiolase with the fluorescent thiol reagent S-mercurio-N-dansyl-L-cysteine. Biochemistry. 1982 Nov 23; 21(24):6112-8. PMID: 6129889.
      View in: PubMed
    67. Gilbert HF. Biological disulfides: the third messenger? Modulation of phosphofructokinase activity by thiol/disulfide exchange. J Biol Chem. 1982 Oct 25; 257(20):12086-91. PMID: 6214556.
      View in: PubMed
    68. Gilbert HF. Proton transfer from acetyl-coenzyme A catalyzed by thiolase I from porcine heart. Biochemistry. 1981 Sep 29; 20(20):5643-9. PMID: 6117316.
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    69. Gilbert HF, Lennox BJ, Mossman CD, Carle WC. The relation of acyl transfer to the overall reaction of thiolase I from porcine heart. J Biol Chem. 1981 Jul 25; 256(14):7371-7. PMID: 6114098.
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    70. Gilbert HF, Stewart MD. Inactivation of hydroxymethylglutaryl-CoA reductase from yeast by coenzyme A disulfide. J Biol Chem. 1981 Feb 25; 256(4):1782-5. PMID: 7007371.
      View in: PubMed
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